| 白眉蝮蛇蛇毒及蛇毒酶的激光解吸飞行时间质谱研究 |
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| 引用本文： | 孙明忠,刘志强,丁兰,赵大庆,刘淑莹,倪嘉缵. 白眉蝮蛇蛇毒及蛇毒酶的激光解吸飞行时间质谱研究[J]. 高等学校化学学报, 2000, 21(4): 538-540 |
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| 作者姓名： | 孙明忠 刘志强 丁兰 赵大庆 刘淑莹 倪嘉缵 |
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| 作者单位： | 中国科学院长春应用化学研究所, 长春 130022 |
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| 基金项目： | 吉林省科学技术委员会基金 !(批准号 :962 90 7) |
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| 摘 要： | Perkins等[1]用MALDI/TOF/MS对不同种类蛇毒中神经毒素进行了分析,彭嘉柔等[2,3]对江浙蝮蛇和蛇毒粗组份进行了初步质谱表征.但蛇毒蛋白纯化困难,因此对单一组份的研究较少且不系统.本文以白眉蝮蛇蛇毒(AgkistrodonblomhoffiiUssurensis,ABUV)为原料,纯化得到了精氨酸酯酶(Arginineesterase,AEase)、磷脂酶A2(PhospholipaseA2,PLA2)、纤溶酶(fibrinolyticenzyme)和L-氨基酸氧化酶(L-aminoacidoxidase),并且用MALDI/TOF/MS法对它们和蛇毒粗毒进行了系统研究.1 实验部分1.1 仪器和药品 激光解吸质谱仪为美国Molecular公司L…
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| 关 键 词： | 蛇毒 酶 激光解吸飞行时间质谱 |
| 收稿时间： | 1999-06-24 |
Mass Spectrometry Study on Snake Venoms and Enzymes |
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| SUN Ming-Zhong,LIU Zhi-Qiang,DING Lan,ZHAO Da-Qing,LIU Shu-Ying,NI Jia-Zuan. Mass Spectrometry Study on Snake Venoms and Enzymes[J]. Chemical Research In Chinese Universities, 2000, 21(4): 538-540 |
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| Authors: | SUN Ming-Zhong LIU Zhi-Qiang DING Lan ZHAO Da-Qing LIU Shu-Ying NI Jia-Zuan |
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| Affiliation: | Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China |
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| Abstract: | In this paper, three kinds of snake venoms and four kinds of enzymes (phospholipase A 2, fibrinolytic enzyme, arginine esterase and L amino acid oxidase) isolated from the snake venom were analyzed. As the snake venom was different, the MALDI/TOF/MS showed difference. The MALDI/TOF/MS determination results could be affected by the concentrations of snake venom enzymes. And the mechanisms of desorption and ionization was also given in this study. By using MALDI/TOF/MS we obtained the accurate molecular weights and homogeneities of the enzymes. The apparent characteristics of the positive MALDI/TOF/MS of enzymes composed by two subunits were also given out. The results showed that MALDI/TOF/MS is an effective analytic method for discovering new components from snake venom complexes. And it is reliable to use this method to determine the molecular weights and purifies of protein molecules. [WT5HZ] |
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| Keywords: | Snake venoms Enzymes MALDI/TOF/MS |
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