| 四川老坛泡菜中乳酸菌的分离与鉴定 |
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| 引用本文： | 潘 晴 曾小群 彭刘杨 曾志丹 潘道东 吴 振. 四川老坛泡菜中乳酸菌的分离与鉴定[J]. 宁波大学学报(理工版), 2017, 0(2): 22-28 |
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| 作者姓名： | 潘 晴 曾小群 彭刘杨 曾志丹 潘道东 吴 振 |
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| 作者单位： | 宁波大学 海洋学院, 浙江 宁波 315211 |
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| 摘 要： | 老坛泡菜比一般泡菜具有更好的菌种多样性和菌群稳定性. 为筛选乳酸菌菌株, 从四川省眉山市仁寿县新安村农家坛龄达30年以上的老坛泡菜中, 分离得到12株疑似乳酸菌, 进行形态学观察、生理生化实验鉴定, 进一步利用16S rRNA基因序列比对、构建系统发育树, 确定疑似乳酸菌菌种. 分离鉴定出5株植物乳杆菌、3株短乳杆菌、2株肠膜明串珠菌、1株干酪乳杆菌和1株屎肠球菌, 其中以植物乳杆菌数量最多.
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| 关 键 词： | 老坛 泡菜 乳酸菌 分离 鉴定 |
Isolation and identification of lactic acid bacteria from old jar pickles in Sichuan province |
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| PAN Qing,ZENG Xiao-qun,PENG Liu-yang,ZENG Zhi-dan,PAN Dao-dong,WU Zhen. Isolation and identification of lactic acid bacteria from old jar pickles in Sichuan province[J]. Journal of Ningbo University(Natural Science and Engineering Edition), 2017, 0(2): 22-28 |
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| Authors: | PAN Qing ZENG Xiao-qun PENG Liu-yang ZENG Zhi-dan PAN Dao-dong WU Zhen |
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| Affiliation: | School of Marine Sciences, Ningbo University, Ningbo 315211, China |
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| Abstract: | The stability and diversity of bacteria from the pickles marinated in old jars are better than those in normal jars. In the current study, the old-jar pickles were more than 30 years old and obtained from Xin-an Village, Renshou County, Meishan City, Sichuan Province. The pickles were used to screen lactic acid bacteria (LAB) with good properties. Twelve strains of LAB were isolated from the old-jar pickles and were identified by morphological observation, physiological and biochemical test, 16S rRNA gene sequence alignment and phylogenetic analysis. Five strains of Lactobacillus plantarum, 3 strains of Lactobacillus brevis, 2 strains of Leuconostoc mesenteroides, 1 strain of Lactobacillus casei and 1 strain of Enterococcus faecium were isolated and identified as predominant bacteria, while Lactobacillus plantarum was dominant among these 5 species of LAB. |
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| Keywords: | old jar pickle lactic acid bacteria separation identification |
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