| 微流控芯片单细胞进样和溶膜 |
| |
| 引用本文： | 高健,殷学锋,方肇伦,夏方诠. 微流控芯片单细胞进样和溶膜[J]. 高等学校化学学报, 2003, 24(9): 1582-1584 |
| |
| 作者姓名： | 高健 殷学锋 方肇伦 夏方诠 |
| |
| 作者单位： | 浙江大学化学系,微分析系统研究所,杭州,310027;山东大学化学系 |
| |
| 基金项目： | 国家自然科学基金(批准号:20299030) |
| |
| 摘 要： | 单细胞分析对重大疾病的早期诊断、治疗和药物筛选以及细胞生理、病理过程的研究有重要意义.将毛细管电泳用于单细胞多组分的测定已取得一些成果,但受毛细管的一维结构限制,单细胞进样和溶膜操作较复杂.微流控分析芯片的网络结构和微米级的通道尺寸使简化单细胞分析成为可能.
|
| 关 键 词： | 微流控芯片 单细胞 装样 溶膜 |
| 文章编号： | 0251-0790(2003)09-1582-03 |
| 收稿时间： | 2003-04-18 |
Single Cell Injection and Lysis on a Microfluidic Chip |
| |
| GAO Jian,YIN Xue-Feng,FANG Zhao-Lun,XIA Fang-Quan. Single Cell Injection and Lysis on a Microfluidic Chip[J]. Chemical Research In Chinese Universities, 2003, 24(9): 1582-1584 |
| |
| Authors: | GAO Jian YIN Xue-Feng FANG Zhao-Lun XIA Fang-Quan |
| |
| Affiliation: | Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310027, China |
| |
| Abstract: | A microfluidic system was developed for the analysis of single biological cells, with functional integration of cell sampling, single cell loading, docking, lysing, and capillary electrophoretic separation in microfabricated channels on a single glass chip. Channels were 12 μm deep and 75μm wide, with a double-Tdesign cell injector that was directly connected to a capillary electrophoretic separation channel with an effective separation length of 37 mm. During sampling with a cell suspension (cell population 1. 2×105cells/mLin physiological salt solution), differential hydrostatic pressure (created by adjusting liquid levels in the four reservoirs) was used to control cell flow exclusively through the 200 μm channel between the two T-junctions. Single cell loading into the separation channel was achieved by electrophoretic means by applying a set of potentials at the four reservoirs, counteracting the hydrostatic flow. Aspecial docking (adhering) procedure for the loaded cell was applied through flow control to affect cell lysis at the applied CE separation voltage(1. 4 kV) within the working electrolyte(pH 9. 2 borate buffer) without additional lysates. The docked lysing approach reduced dispersion of released intracellular constituents, and significantly improved the CEseparation efficiency. FITC-labeled components in the cellular membrane of single human erythrocyte cells were detected by using laser induced fluorescence. Aretention time precision of 0. 9% RSD(n=4) for FITC, and an average separation efficiency of 18 μm plate height for FITCwere achieved. |
| |
| Keywords: | Microfluidic chip Single cell Loading Lysing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《高等学校化学学报》浏览原始摘要信息 |
|
点击此处可从《高等学校化学学报》下载全文 |
|
