The Biosynthesis and Catabolism of the Maleic Anhydride Moiety of Stipitatonic Acid |
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| Authors: | Dr. Ahmed al Fahad Amira Abood Prof. Dr. Thomas J. Simpson Prof. Dr. Russell J. Cox |
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| Affiliation: | 1. School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS (UK);2. Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Cairo (Egypt);3. Institut für Organische Chemie, Leibniz Universit?t Hannover, Schneiderberg 1B, 30167 Hannover (Germany) |
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| Abstract: | A series of directed knockout experiments, combined with an in vitro assay of pathway components, has elucidated for the first time the chemical steps involved in the biosynthesis of the tropolone class of fungal maleic anhydrides. The pathway involves the stepwise oxidation of aldehyde and methyl carbon atoms to form a 1,2‐dicarboxylate. A hydrolase‐catalyzed interconversion of this and the corresponding maleic anhydride, followed by decarboxylation of the diacid leads to the pathway’s final product of stipitatic acid. |
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| Keywords: | biosynthesis fungi maleic anhydride tropolones |
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