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| HBV X蛋白与宿主APOBEC3G蛋白之间的相互作用 | |
| 引用本文： | 高婧,董长垣,胡俊,李艳,Joseph K K Li,戴莹,郭淑芳.HBV X蛋白与宿主APOBEC3G蛋白之间的相互作用[J].武汉大学学报(理学版),2007,53(4):471-474. |
| 作者姓名： | 高婧 董长垣 胡俊 李艳 Joseph K K Li 戴莹 郭淑芳 |
| 作者单位： | 1. 武汉大学,医学病毒学研究所/病毒学国家重点实验室,湖北,武汉,430071 2. 武汉大学,人民医院,湖北,武汉,430071 3. 犹他州立大学生物系,犹他,洛根,84322 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 将原核重组质粒双酶切后释放的χ基因与真核表达载体连接，构建真核重组质粒pcDNA3.1-X，然后与真核重组质粒pcDNA3.1-APOBEC3G共转染HepG2细胞，提取蛋白进行免疫共沉淀并用Western blot分析结果。免疫共沉淀后，HA-APOBEC3G融合蛋白能够在抗HBx的免疫沉淀物中检测出来，在抗HA标签的免疫沉淀物中也能检测到HBx。揭示了这两种蛋白能在受体细胞内形成复合物。结果表明乙肝病毒X蛋白与APOBEC3G蛋白在细胞内能发生相互作用，提示APOBEC3G抗HBV作用可能与乙肝病毒X蛋白有关。 |
| 关 键 词： | 乙型肝炎病毒 X蛋白 免疫共沉淀 |
| 文章编号： | 1671-8836（2007）04-0471-04 |
| 修稿时间： | 2007-04-16 |
Interaction Between HBV X Protein and Human APOBEC3G Protein |
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| GAO Jing,DONG Changyuan,HU Jun,LI Yan,Joseph K K Li,DAI Ying,GUO Shufang.Interaction Between HBV X Protein and Human APOBEC3G Protein[J].JOurnal of Wuhan University:Natural Science Edition,2007,53(4):471-474. | |
| Authors: | GAO Jing DONG Changyuan HU Jun LI Yan Joseph K K Li DAI Ying GUO Shufang |
| Institution: | 1. School of Medicine, Wuhan University/ State Key Laboratory of Virology, Wuhan 430071, Hubei, China; 2. Renmin Hospital, Wuhan University, Wuhan 430071, Hubei, China; 3. Department of Biology, Utah State University, Logan UT 84322, USA |
| Abstract: | To investigate the interaction between HBV X protein and Human APOBEC3G protein, then to discover the mechanism of its antiviral activity in another perspective. The X gene was digested from prokaryotic expressing plasmid, and ligated with eukaryotic expressing vector. Then HepG2 cells cotransfected with recombinant plasmid pcDNA3.1-X and pcDNA3.1-APOBEC3G. The interaction between HBx and APOBEC3G protein was detected by co-immunoprecipitation. We construct eukaryotic expressing vector with X gene successfully. The HBx and HA-APOBEC3G fusion protein were detected respectively in the immunoprecipitation by anti HBx and anti HA-tag. It indicates that the two proteins can form compounds in HepG2 cells. X protein can combine with APOBEC3G protein in vivo. It suggests HBx plays a role in the effect of anti-human hepatitis B virus of APOBEC3G. |
| Keywords: | APOBEC3G |
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