| 微量热法研究Na+,K+-ATPase酶促反应 |
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| 引用本文： | 李霞,三志勇,谢修银,张文婷,汪存信.微量热法研究Na+,K+-ATPase酶促反应[J].武汉大学学报(理学版),2002,48(4):397-400. |
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| 作者姓名： | 李霞 三志勇 谢修银 张文婷 汪存信 |
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| 作者单位： | 武汉大学化学与分子科学学院,湖北,武汉,430072 |
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| 基金项目： | 国家自然科学基金资助项目 (3 0 0 70 2 0 0 ) |
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| 摘 要： | 首次用微量热方法研究了Na^ ,K^ -ATPase催化ATP水解的酶促反应，在310．15K，PH＝7．4的100mmol.L^-1Tris-HCl,100mmol.L^-1NaCl,10mmol.L^-1KCl,3mmol.L^-1MgCl2缓冲溶液环境下，用对比进度法得出了Na^ ,K^ -ATPase酶促反应的米氏常数Km值和最大反应速率Vm值，所得常数值与文献给出的相符，并用比色法得出了一致的Km和Vm值，同时初步研究了酶预稀释对酶促反应的影响。
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| 关 键 词： | 微量热 Na^＋ K^＋－ATPase 酶促反应 比色法 |
| 文章编号： | 0253-9888(2002)04-0397-04 |
| 修稿时间： | 2002年2月11日 |
Microcalorimetric Studies on Na+,K+-ATPase |
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| LI Xia,WANG Zhi\|yong,XIE Xiu\|ying,ZHANG Wen\|ting,WANG Cun\|xin.Microcalorimetric Studies on Na+,K+-ATPase[J].JOurnal of Wuhan University:Natural Science Edition,2002,48(4):397-400. |
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| Authors: | LI Xia WANG Zhi\|yong XIE Xiu\|ying ZHANG Wen\|ting WANG Cun\|xin |
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| Abstract: | For the first time, the hydrolysis reaction of ATP catalyzed by Na + ,K + \|ATP ase was studied with microcalorimetry. The Micheal Constant( K m ) and Maximal Velocity( V m ) of Na + , K + \|ATP ase under certain experimental conditions were determined by reduced extent method. The constants obtained above were in good coincidence with literature figure. Identical values of K m and V m were obtained by colorimetric determination of inorganic phosphate. The effect of excessive dilution on enzyme-catalyzed reaction was also investigated. |
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| Keywords: | Na + K + \| ATP ase enzyme\|catalyzed reaction microcalorimetry colorimetry |
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