| NaCl对水稻谷氨酰胺合成酶活性及同工酶的影响 |
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| 引用本文： | 李常健,林清华,张楚富,李泽松,彭进,朱英国,PengShaobing,BennettJohn.NaCl对水稻谷氨酰胺合成酶活性及同工酶的影响[J].武汉大学学报(理学版),1999(4). |
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| 作者姓名： | 李常健 林清华 张楚富 李泽松 彭进 朱英国 PengShaobing BennettJohn |
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| 作者单位： | 武汉大学生命科学学院!武汉430072，武汉大学生命科学学院!武汉430072，武汉大学生命科学学院!武汉430072，武汉大学生命科学学院!武汉430072，武汉大学生命科学学院!武汉430072，武汉大学生命科学学院!武汉430072，InternationalRiceResearchInstitute!P.O.Box3127，1271MakatiCity，P |
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| 基金项目： | 国家自然科学基金,湖北省自然科学基金,国际水稻研究所资助项目 |
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| 摘 要： | 在营养液中 Ｎａ Ｃｌ的胁迫下，水稻根和叶的谷氨酰胺合成酶（ Ｇ Ｓ）的活性降低，但叶的 Ｇ Ｓ活性对 Ｎａ Ｃｌ浓度的敏感性大于根根和叶中的 Ｇ Ｓ活性变化与根和叶的平均质量和可溶性蛋白水平的变化是一致的 Ｎａｔｉｖｅ Ｐ Ａ Ｇ Ｅ活性染色以及 Ｉｍ ｍ ｕｎｏｂｌｏｔｔｉｎｇ 检测表明，根和叶的 Ｇ Ｓ活性的降低主要是由于 Ｇ Ｓｒｂ 和 Ｇ Ｓ２的活性及其相应蛋白质水平降低所致
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| 关 键 词： | 谷氨酰胺合成酶 同工酶 水稻 NaCl |
Effect of NaCl Stress on Activity and Isozymes of Glutamine Synthetase in Rice Plants |
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| LI Chang jian ,LIN Qing hua ,ZHANG Chu fu ,LI Ze song ,PENG Jin ,ZHU Ying guo ,PENG Shao bing ,BENNETT John.Effect of NaCl Stress on Activity and Isozymes of Glutamine Synthetase in Rice Plants[J].JOurnal of Wuhan University:Natural Science Edition,1999(4). |
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| Authors: | LI Chang jian LIN Qing hua ZHANG Chu fu LI Ze song PENG Jin ZHU Ying guo PENG Shao bing BENNETT John |
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| Institution: | LI Chang jian 1,LIN Qing hua 1,ZHANG Chu fu 1,LI Ze song 1,PENG Jin 1,ZHU Ying guo 1,PENG Shao bing 2,BENNETT John 2 |
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| Abstract: | The activity of glutamine synthetase (GS) in rice roots and leaves was decreased under NaCl stress in nutrient solution, but the activity of GS in Leaves was more sensitive to NaCl stress than that in roots.The change of GS activity in rice roots and leaves was consistent with the change of the biomass and soluble proteins in rice roots and leaves. The Determination of GS by Native PAGE, activity staining,and immunoblotting showed that the decline of GS activity of rice roots and leaves was mainly due to the reduction of the activity and protein levels of GSrb in roots and GS2 in leaves, respectively. |
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| Keywords: | glutamine synthetase isozyme rice NaCl |
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