| 脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化 |
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| 引用本文： | 王艳芳,王杰,宋锡瑾.脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化[J].浙江大学学报(理学版),2004,31(5):557-559. |
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| 作者姓名： | 王艳芳 王杰 宋锡瑾 |
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| 作者单位： | 1. 浙江大学,化学系,浙江,杭州,310028
2. 浙江大学,制药工程研究所,浙江,杭州,310027 |
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| 摘 要： | 研究了聚乙二醇二丙烯酸酯(PEGDA)作为预聚物,三羟甲基丙烷三丙烯酸酯(TMPTA)为交联剂,紫外光引发交联聚合,实现对脂肪酶固定化的方法.结果表明所制备的固定化酶与游离酶相比催化活性提高了8倍以上,且其稳定性及对环境的适应性也大大提高,在95%的乙醇中浸泡5 d后,其催化活力仍有96%之多.
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| 关 键 词： | 固定化 脂肪酶 载体 |
| 文章编号： | 1008-9497(2004)05-557-03 |
| 修稿时间： | 2004年3月3日 |
Immobilization of lipase using the polymer of polyglycol diacrylate as carrier |
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| WANG Yan-fang,WANG Jie,SONG Xi-jin.Immobilization of lipase using the polymer of polyglycol diacrylate as carrier[J].Journal of Zhejiang University(Sciences Edition),2004,31(5):557-559. |
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| Authors: | WANG Yan-fang WANG Jie SONG Xi-jin |
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| Institution: | WANG Yan-fang~1,WANG Jie~1,SONG Xi-jin~2 |
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| Abstract: | The immobilization of lipase using polymer as carrier was studied.The polyglycol diacrylate (PEGDA)crosslinked with TMPTA were synthesized by UV-induced polymerization to make lipase carrier. The result showed that this polymer is an effective carrier, lipase is immobilized on this carrier,its activities were improved more than 8 times and 96% activity of immobilized lipase is retained after it is soaked in ethanol(95%) for 5 days. |
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| Keywords: | imm obilization lipase carrier |
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