| 用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究 |
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| 引用本文： | 明镇寰,岳春梅.用PCR技术检测生物硝化池污水中硝化细菌(Nitrobacteria)的研究[J].浙江大学学报(理学版),1999,26(2):83-86. |
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| 作者姓名： | 明镇寰 岳春梅 |
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| 基金项目： | 浙江省科委(971103079)、浙江省教委和浙江省环保局资助项目 |
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| 摘 要： | 从生物硝化池污水水样中分离了硝化细菌DNA，以其为模板，针对其16Sr RNA基因及23Sr RNA基因而设计合成了两对引物并分别进行了PCR扩增，扩增产物的2%琼脂糖凝胶电泳结果显示，硝化细菌DNA模板得到了特异性扩增．参照分子量标准（Marker）的电泳结果，被扩增的DNA条带大小分别约为400bp和730bp．实验结果表明PCR 技术可用于污水中硝化细菌的快速检测．
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| 关 键 词： | 硝化细菌 污水 多聚酶链式反应 |
| 收稿时间： | 1998-06-29 |
Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis. |
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| MING Zhen-huan,YUE Chun-mei,XU Bing-sen,et al..Detection of Nitrobacteria Populations in Wastewater of Biological Nitrification Stage by Polymerase Chain Reaction Analysis.[J].Journal of Zhejiang University(Sciences Edition),1999,26(2):83-86. |
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| Authors: | MING Zhen-huan YUE Chun-mei XU Bing-sen |
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| Institution: | College of Life Science,Zhejiang University,Hangzhou 310012,China |
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| Abstract: | Nitrobacteria DNA was extracted from wastewater of biological nitrification stage and two PCR primers based on the DNA sequences of 16S r DNA and 23S r DNA were synthesized and used for PCR amplification. The results showed that Nitrobacteria DNA was amplified specifically and the size of amplified DNA fragments were about 400 bp and 730 bp respectively. It is suggested that PCR analysis can be used for the rapid detection of Nitrobacteria in wastewater. |
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| Keywords: | Nitrobacteria wastewater PCR |
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