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| 水中铜绿微囊藻定量PCR检测方法的建立 | |
| 引用本文： | 朱金余,李海波,陈芳,陈红.水中铜绿微囊藻定量PCR检测方法的建立[J].浙江大学学报(理学版),2013,40(2):211-215. |
| 作者姓名： | 朱金余 李海波 陈芳 陈红 |
| 作者单位： | 1. 浙江大学环境工程研究所,浙江杭州,310058 2. 浙江省林业科学研究院,浙江杭州,310023 3. 杭州市环境监测中心站,浙江杭州,310007 |
| 基金项目： | 水体污染控制与治理科技重大专项"苕溪上游河口与滨岸带营养物质生态拦截示范工程" |
| 摘 要： | 为了建立一种检测水中铜绿微囊藻浓度的定量PCR方法,分别用SDS裂解法和DNeasy Blood & Tissue Kit试剂盒提取标准品的DNA并建立标准曲线.结果表明采用DNeasy Blood & Tissue Kit试剂盒提取的标准品DNA具有很好的重复性,经定量PCR扩增得到的标准曲线R2为0.968,可以用来检测样品中铜绿微囊藻的浓度,为建立一套长期有效的测定水中铜绿微囊藻浓度的监测体系提供了技术参考. |
| 关 键 词： | 实时荧光定量PCR 铜绿微囊藻 DNA提取方法 |
| 收稿时间： | 2011-12-02; |
Applications of real-time polymerase chain reaction for quantification of Microcystis aeraginosa |
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| ZHU Jin-yu , LI Hai-bo , CHEN Fang , CHEN Hong.Applications of real-time polymerase chain reaction for quantification of Microcystis aeraginosa[J].Journal of Zhejiang University(Sciences Edition),2013,40(2):211-215. | |
| Authors: | ZHU Jin-yu LI Hai-bo CHEN Fang CHEN Hong |
| Institution: | 1. Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; 2. Zhejiang Forestry Academy, Hangzhou 310023, Chinas 3. Hangzhou Environmental Mornitoring Central Station, Hangzhou 310007, China) |
| Abstract: | SDS lysis method and DNeasy Blood ＆ Tissue Kit method were compared to establish an appropriate standard curve for the detection of Microcystis aeraginosa. A high correlation standard curve of the standard solution DNA extracted by DNeasy Blood ＆Tissue Kit was established（R^2=0. 968）,this result above provide a new method for quantitating the Microcystis in lakes. |
| Keywords: | real-time quantitative PCR Microcystis aeraginosa~ DNA extraction |
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