| 鲫鲤杂交F1及其亲本RAPD标记 |
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| 引用本文： | 刘必谦,王亚军,周湘池.鲫鲤杂交F1及其亲本RAPD标记[J].宁波大学学报(理工版),2000,13(4):31-34. |
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| 作者姓名： | 刘必谦 王亚军 周湘池 |
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| 作者单位： | 宁波大学生命科学与生物工程学院，浙江宁波 315211 |
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| 摘 要： | 用RAPD技术对红鲫(♀)×野鲤(♂)及其F1个体进行了研究．15个引物在鲤鱼中扩增出86条带(33条具多态性)，红鲫75条带(24条呈多态性)，两者共享25条(16条呈多态性)．F
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| 关 键 词： | 野鲤 红鲫 随机扩增多态DNA（RAPD） 杂交 |
| 文章编号： | 1001-5132(2000)04-0031-04 |
| 修稿时间： | 2000年6月16日 |
RAPD Marker Study in F1 Hybrid [Red Crucian Carp(♀)× Carp(♂)] and Its Parents |
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| LIU Bi-qian,WANG Ya-jun,ZHOU Xiang-chi.RAPD Marker Study in F1 Hybrid [Red Crucian Carp(♀)× Carp(♂)] and Its Parents[J].Journal of Ningbo University(Natural Science and Engineering Edition),2000,13(4):31-34. |
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| Authors: | LIU Bi-qian WANG Ya-jun ZHOU Xiang-chi |
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| Abstract: | RAPD technique was used to study red crucian carp, carp and their F1 hybrid. With 15 primers, 86 bands (33 were Polymorphism) were obtained in carps, 75 bands (24 were polymorphism) in red crucian carps and 92 bands (35 were polymorphism) in F1 hybrid. Carp and red crucian carp shared 15 bands. All the bands of parents could be found in the hybrid individuals. RAPD markers are dominant markers. Heterozygous bands in parents segregated in F, individuals. RAPD technique is useful for the identification of hybrid individuals and analysis of heterozygous loci. |
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| Keywords: | carp red crucian carp random amplified polymorphism DNA(RAPD) hybridization |
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