| 嗜酸乳杆菌发酵产亚油酸异构酶培养基条件的研究 |
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| 引用本文： | 石清花,翁佩芳,吴祖芳.嗜酸乳杆菌发酵产亚油酸异构酶培养基条件的研究[J].宁波大学学报(理工版),2006,19(4):457-461. |
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| 作者姓名： | 石清花 翁佩芳 吴祖芳 |
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| 作者单位： | 宁波大学,生命科学与生物工程学院,浙江,宁波,315211 |
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| 基金项目： | 浙江省自然科学基金;浙江省宁波市博士学科点专项科研基金;宁波大学校科研和教改项目 |
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| 摘 要： | 以产亚油酸异构酶为指标,鉴定从泡菜中分离的嗜酸乳杆菌L1,对产酶培养基的碳源、氮源、无机盐及其配比和表面活性剂等方面进行了优化,最后得到了优化后培养基,其组成为:蛋白胨10g,酵母提取物5g,牛肉膏15g,葡萄糖20g,无水乙酸钠2.5g,柠檬酸二铵3g,Tween-801.5mL,MgSO40.14g,MnSO4.H2O0.3g,K2HPO4.3H2O1.31g.经发酵验证:培养基经优化后产酶是未优化前的1.32倍。
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| 关 键 词： | 共轭亚油酸 亚油酸异构酶 培养基 优化 |
| 文章编号： | 1001-5132(2006)04-0457-05 |
| 收稿时间： | 2006-07-26 |
| 修稿时间： | 2006年7月26日 |
Optimization of a Fermentation Medium for Linoleic Acid Isomerase Production with Lactobacillus acidophilus |
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| SHI Qing-hua,WENG Pei-fang,WU Zu-fang.Optimization of a Fermentation Medium for Linoleic Acid Isomerase Production with Lactobacillus acidophilus[J].Journal of Ningbo University(Natural Science and Engineering Edition),2006,19(4):457-461. |
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| Authors: | SHI Qing-hua WENG Pei-fang WU Zu-fang |
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| Abstract: | A type of Lactobacillus acidophilus L1,isolated from pickled vegetable,was used as the benchmark for linoleic acid isomerase production.The medium components and concentration such as carbon source,nitro source,inorganic salt and surfactant,etc.were tested with linoleic acid isomerase as an indicator.At last,the optimum medium as well as its concentration was obtained,which has the formulation of(per liter) peptone 10 g,yeast extract 5 g,beef extract 15 g,glucose 20 g,sodium acetate anhydrous 2.5 g,ammonium citrate dibasic 3 g,Tween-80 1.5 mL,MgSO4 0.14 g,MnSO4.H2O 0.3 g and K2HPO4.3H2O 1.31 g.The enzymatic activity unit of linoleic acid isomerase is found to be 1.32 times those values without optimization. |
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| Keywords: | conjugated linoleic acid linoleic acid isomerase medium optimization |
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