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| 伊红和牛血清白蛋白相互作用 | |
| 引用本文： | 石燕,欧阳萌,郑燚.伊红和牛血清白蛋白相互作用[J].南昌大学学报(理科版),2004,28(4):400-404. |
| 作者姓名： | 石燕 欧阳萌 郑燚 |
| 作者单位： | 1. 南昌大学 食品科学教育部,重点实验室,江西,南昌,330027 2. 南昌大学 分析测试中心,江西,南昌,330047 3. 南昌大学 江西师范大学,江西,南昌,330027 |
| 基金项目： | 南昌大学2003年度校科研基金资助项目(Z02478) |
| 摘 要： | 运用紫外光谱法和荧光光谱法研究了伊红和牛血清白蛋白(以下简称BSA)的相互作用。结果表明，伊红是与BSA中特定部位(弱极性区域)相结合，且符合Scatchard模型。 |
| 关 键 词： | 伊红 牛血清白蛋白 紫外光谱法 荧光光谱法 |
| 文章编号： | 1006-0464(2004)04-0400-05 |
| 修稿时间： | 2003年3月9日 |
STUDY ON THE INTERACTION BETWEEN EOSIN AND BOVINE SERUM ALBUMIN |
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| SHI Yan,OU Yang-meng,ZHENG Yi.Centre of Annlysis and Tesing,Nanchang University,Nanchang ,China, .Jiangxi Normal University,Nanchang Jiangxi ,China.STUDY ON THE INTERACTION BETWEEN EOSIN AND BOVINE SERUM ALBUMIN[J].Journal of Nanchang University(Natural Science),2004,28(4):400-404. | |
| Authors: | SHI Yan OU Yang-meng ZHENG YiCentre of Annlysis and Tesing Nanchang University Nanchang China Jiangxi Normal University Nanchang Jiangxi China |
| Institution: | SHI Yan~1,OU Yang-meng~2,ZHENG Yi~32.Centre of Annlysis and Tesing,Nanchang University,Nanchang 330047,China, 3.Jiangxi Normal University,Nanchang Jiangxi 330027,China) |
| Abstract: | The interaction between Eosin and Bovine Serum Albumin(BSA) had been studied by absorption spectrometry and fluorescence spectrometry.The conformational change of BSA due to the addition of Eosin was discussed.The results of UV-absorption spectrometry show that the way of the Interaction Between Eosin and BSA should obey to the Scatchard Model,that is,Eosin should be combine to some certain regions of BSA.Meanwhile,taking advantage of fluorescence quenching,the reaction mechanism of Eosin and BSA was explored. |
| Keywords: | eosin bovine serum albumin UV-absorption spectrometry fluorescence spectrometry interaction |
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