| 脑缺血再灌流后大鼠海马锥体细胞和星形胶质细胞的体视学 |
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| 引用本文： | 辜清,姚立希,吴娣,万勇,杨琳.脑缺血再灌流后大鼠海马锥体细胞和星形胶质细胞的体视学[J].南昌大学学报(理科版),2009,33(2). |
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| 作者姓名： | 辜清 姚立希 吴娣 万勇 杨琳 |
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| 作者单位： | 南昌大学,生命科学学院,江西,南昌,330031 |
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| 摘 要： | 为了研究脑缺血再灌流后海马锥体细胞和星形胶质细胞的变化,选取24只SD大鼠,随机分为对照组、缺血再灌流后6、12、24 h组,采用Nissl染色,GFAP免疫组织化学方法并应用体视学参数分别对海马CA1、CA3区锥体细胞和星形胶质细胞进行形态学定量分析.结果显示,海马CA1、CA3区锥体细胞体积密度(Vv)和表面积密度(Sv)随着缺血再灌流后存活时间的延长而减小,与对照组相比,6 h组显著降低(P<0.05),12 h组和24 h组极显著降低(P<0.01);而海马区星型胶质细胞的Vv、Sv却随缺血再灌流后存活时间的延长而加大,与对照组相比,6 h组无显著性差异(P>0.05),12、24 h组具极显著差异(P<0.01,P<0.01);同组海马CA1和CA3区,以及同组同一海马区域左右两侧比较,锥体细胞和星形胶质细胞Vv、Sv均无显著性差异(P>0.05).以上结果表明,海马CA1、CA3区锥体细胞随着缺血再灌流时间的延长受损加重,而星形胶质细胞功能趋于活跃且与锥体细胞损伤密切相关.
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| 关 键 词： | 脑缺血再灌流 海马 锥体细胞 星形胶质细胞 体视学 |
The Stereological Study on Pyramidal Cells and Astrocytes in Rat Hippocampus after Cerebral Ischemia-Reperfusion |
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| GU Qing,YAO Li-xi,WU Di,WAN Yong,YANG Lin.The Stereological Study on Pyramidal Cells and Astrocytes in Rat Hippocampus after Cerebral Ischemia-Reperfusion[J].Journal of Nanchang University(Natural Science),2009,33(2). |
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| Authors: | GU Qing YAO Li-xi WU Di WAN Yong YANG Lin |
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| Institution: | College of Life Sciences;Nanchang University;Nanchang 330031;China |
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| Abstract: | To investigate the changes of pyramidal cells and astrocytes in rat hippocampus after ischemia-reperfusion,24 SD rats were randomly divided into four groups which were control group,ischemia-reperfusion 6 h group,12 h group and 24 h group.Changes in hippocampus CA1,CA3 were observed by Nissl staining and anti-GFAP immunohistochemistry,stereological method was used to evaluate changes as well.The results showed that the volumn density(Vv) and surface density(Sv) of pyramidal cell in CA1 and CA3 decreased as ... |
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| Keywords: | cerebral ischemia-reperfusion hippocampus pyramidal cells astrocytes stereology |
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