| 光谱法研究刺芒柄花素与牛血清白蛋白的相互作用 |
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| 引用本文： | 倪永年,张方圆,张秋兰.光谱法研究刺芒柄花素与牛血清白蛋白的相互作用[J].南昌大学学报(理科版),2011,35(2). |
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| 作者姓名： | 倪永年 张方圆 张秋兰 |
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| 作者单位： | 1. 南昌大学食品科学与技术国家重点实验室,江西,南昌,330047;南昌大学化学系,江西,南昌,330031
2. 南昌大学化学系,江西,南昌,330031 |
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| 基金项目： | 国家自然科学基金资助项目(21065007); 南昌大学食品科学与技术国家重点实验室基金资助项目(SKLF-MB-200807 and SKLF-TS-200919) |
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| 摘 要： | 在生理pH7.4条件下,应用光谱法研究药物小分子刺芒柄花素与牛血清白蛋白相互作用机理。通过荧光法和紫外吸收光谱法确定了刺芒柄花素对牛血清白蛋白的荧光猝灭机制。采用Stern-Volmer方程求出相互作用的猝灭常数,双对数方程求出结合常数Ka和结合位点数n,进而利用热力学公式判别作用力类型。结果表明:刺芒柄花素与牛血清白蛋白的相互作用的荧光猝灭属于静态方式,298 K时结合常数Ka为1.39×106L.mol-1,结合位点数为1,而主要作用力类型是静电作用。用紫外与同步荧光光谱法研究了刺芒柄花素对BSA构象的影响。
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| 关 键 词： | 刺芒柄花素 牛血清白蛋白 相互作用 荧光光谱 紫外吸收光谱 |
Interaction between Formononetin and bovine serum albumin by spectroscopy |
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| NI Yong-nian,ZHANG Fang-yuan,ZHANG Qiu-lan.Interaction between Formononetin and bovine serum albumin by spectroscopy[J].Journal of Nanchang University(Natural Science),2011,35(2). |
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| Authors: | NI Yong-nian ZHANG Fang-yuan ZHANG Qiu-lan |
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| Institution: | NI Yong-niana,b*,ZHANG Fang-yuanb,ZHANGQiu-lana,b(a.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China,b.Department of Chemistry,Nanchang 330031,China) |
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| Abstract: | The interaction between formononetin and bovine serum albumin(BSA) was studied under physiological condition by spectroscopic method.The quenching mechanism of the fluorescence of BSA by formononetin was studied with the fluorescence and UV-vis spectrosmetry.The binding constants,number of binding sites and types of the binding force were evaluated by using the Stern-Volmer,double-reciprocal and thermodynamic equations.It was found that fluorescence quenching of the interaction was due to the static quenchi... |
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| Keywords: | Formononetin bovine serum albumin interaction fluorescence and UV-vis spectra |
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