Electrospun microfibrous poly(styrene-alt-maleic anhydride)/poly(styrene-co-maleic anhydride) mats tailored for enzymatic remediation of waters polluted by endocrine disruptors |
| |
Authors: | M Ignatova O Stoilova N Manolova DG Mita N Diano C Nicolucci I Rashkov |
| |
Institution: | a Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 103A, 1113 Sofia, Bulgaria b Institute of Genetics and Biophysics of CNR, Via Pietro Castellino, 111-80131 Naples, Italy c Department of Experimental Medicine, Second University of Naples, Via S. M. di Costantinopoli 16, 80138 Naples, Italy d National Institute of Biosystems and Biostructures (INBB), Via le Medaglie d’Oro, 305, 00136 Rome, Italy |
| |
Abstract: | Novel bicomponent microfibrous mats containing targeted amount of reactive maleic anhydride groups were prepared by electrospinning of mixed solutions of poly(styrene-alt-maleic anhydride) and poly(styrene-co-maleic anhydride). Then, amino-functionalized P(St-alt-MA)/P(St-co-MA) mats were obtained by reaction with p-phenylenediamine. ATR-FTIR and XPS spectroscopy were used to characterize pristine and modified P(St-alt-MA)/P(St-co-MA) mats. On the next step, laccase from Trametes versicolor was covalently attached onto the modified mats; the average amount of immobilized enzyme was 40 ± 0.7 mg/g mat. The catalytic activity of the immobilized enzyme was studied in respect to bisphenol A (BPA) endocrine disruptor. The optimum activity of the immobilized enzyme was reached at maximum flow rate of 1.3 mL/s. After 90 min the BPA concentration was reduced by 60% and the catalytic activity of microfibrous mats remained stable for about 30 successive reuses. In addition, the relative activity of laccase immobilized on the microfibrous mats was displayed in a broader pH range as compared to that of the free one. |
| |
Keywords: | Electrospinning Immobilized enzyme Styrene-maleic anhydride copolymers Endocrine disruptors |
本文献已被 ScienceDirect 等数据库收录! |
|