Computational Enzyme Design |
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| Authors: | Dr Gert Kiss Dr Nihan Çelebi‐Ölçüm Dr Rocco Moretti Prof Dr David Baker Prof Dr Dr K N Houk |
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| Institution: | 1. Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Dr. East, Los Angeles CA, 90095 (USA);2. Current address: Department of Chemistry, Stanford University, Stanford, CA 94305 (USA);3. Current address: Yeditepe University, Department of Chemical Engineering, Istanbul (Turkey);4. Department of Biochemistry and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195 (USA) |
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| Abstract: | Recent developments in computational chemistry and biology have come together in the “inside‐out” approach to enzyme engineering. Proteins have been designed to catalyze reactions not previously accelerated in nature. Some of these proteins fold and act as catalysts, but the success rate is still low. The achievements and limitations of the current technology are highlighted and contrasted to other protein engineering techniques. On its own, computational “inside‐out” design can lead to the production of catalytically active and selective proteins, but their kinetic performances fall short of natural enzymes. When combined with directed evolution, molecular dynamics simulations, and crowd‐sourced structure‐prediction approaches, however, computational designs can be significantly improved in terms of binding, turnover, and thermal stability. |
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| Keywords: | active‐site design biomolecular catalysis non‐natural reactions protein engineering theozymes |
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