A Green BODIPY‐Based,Super‐Fluorogenic,Protein‐Specific Labelling Agent |
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| Authors: | Yingche Chen Kelvin Tsao Sydney L Acton Prof Jeffrey W Keillor |
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| Institution: | Department of Chemistry, University of Ottawa, Ottawa, ON, Canada |
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| Abstract: | We report the development of YC23, a novel green BODIPY‐based dimaleimide derivative that undergoes a fluorogenic addition reaction (FlARe) with a genetically encodable peptide tag (dC10α) that can be fused to a protein of interest (POI). We also demonstrate the application of this reaction for the fluorogenic labelling of a specific POI in bacterial lysate and in living mammalian cells. |
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| Keywords: | BODIPY fluorogenic probes maleimide protein labelling |
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