Aptamer-based affinity labeling of proteins |
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| Authors: | Jan L Vinkenborg Günter Mayer Michael Famulok |
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| Institution: | University of Bonn, LIMES Institute, Chemical Biology & Med. Chem. Unit c/o Kekulé-Institute for Organic Chemistry and Biochemistry, Gerhard-Domagk-Strasse 1, 53121 Bonn (Germany) http://www.famuloklab.de. |
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| Abstract: | A most able label: Labeled aptamers can be cross-linked to their target structures in a light-dependent and highly specific manner as a result of a new strategy termed aptamer-based affinity labeling (ABAL) of proteins. The aptamer-protein complexes can be enriched in?vitro, from a cellular lysate and from the surface of living cells, opening new ways to study aptamer interactions in biological contexts. |
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| Keywords: | affinity‐based proteomic profiling (ABPP) aptamers cell‐SELEX photo‐affinity labeling proteomics |
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