A thermodynamic investigation on the binding of phenothiazinium dyes azure A and azure B to double stranded RNA polynucleotides |
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Institution: | 1. Pamukkale University, Faculty of Engineering, Department of Chemical Engineering, 20070 Denizli, Turkey;2. Pamukkale University, Faculty of Science, Department of Chemistry, 20017 Denizli, Turkey;3. Pamukkale University, Faculty of Education, Department of CITE, 20017 Denizli, Turkey |
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Abstract: | The thermodynamics of the reactions of the two phenothiazinium dyes azure A and azure B with the three double stranded ribonucleic acids, poly(A).poly(U), poly(C).poly(G), poly(I).poly(C) were investigated using DSC and ITC. The bound dyes stabilized the RNAs against thermal strand separation. The binding of azure A to the RNAs was predominantly enthalpy dominated while the binding of azure B was favoured by both negative enthalpy and favourable entropy changes. Although electrostatic interaction had a significant role in the binding, non-polyelectrolytic forces dominated the binding process. The negative values of heat capacity changes for the binding suggested a substantial hydrophobic contribution to the binding process. The overall binding affinity of both the dyes to the RNAs varied in the order, poly(A).poly(U) > poly(C).poly(G) > poly(I).poly(C). |
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Keywords: | Phenothiazinium dyes Interaction Intercalation RNA Calorimetry Thermodynamics |
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