(Liquid + liquid) equilibria of polymer-salt aqueous two-phase systems for laccase partitioning: UCON 50-HB-5100 with potassium citrate and (sodium or potassium) formate at 23 °C |
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| Institution: | 1. LSRE – Laboratory of Separation and Reaction Engineering, Associate Laboratory LSRE/LCM, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal;2. IBB – Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal;1. Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, UCSI Heights, 56000 Cheras, Kuala Lumpur, Malaysia;2. Discipline of Chemical Engineering, School of Engineering, Monash University Malaysia, 47500 Bandar Sunway, Selangor, Malaysia;3. Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia;4. Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;5. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;6. Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;7. School of Chemical Sciences, Universiti Sains Malaysia, Minden 11800, Malaysia;8. Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;9. Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia;1. Faculty of Chemical Engineering, Babol University of Technology, PO Box, 484, Babol, Iran;2. Chemical Engineering Department, Faculty of Engineering, Shomal University, PO Box 731, Amol, Mazandaran, Iran;1. Processes Engineering Laboratory, State University of Bahia Southwest, Itapetinga, Bahia, Brazil;2. Department of Exact and Natural Sciences, State University of Southwest of Bahia, 45700-000 Itapetinga, BA, Brazil;3. Department of Food Science, Federal University of Lavras, 37200-000, Lavras, MG, Brazil;1. Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan;2. Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia;3. Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Material Science, Yuan Ze University, No. 135 Yuan-Tung Road, Chungli, Taoyuan 320, Taiwan;4. Department of Chemical Engineering, School of Civil and Chemical Engineering, VIT University, Vellore 632014, India;5. Department of Chemical Engineering, Khalifa University of Science and Technology, The Petroleum Institute, Abu Dhabi, United Arab Emirates;6. Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo and Oslo University Hospital, Norway;7. Faculty of Biology, Hanoi National University of Education, Hanoi, Viet Nam;8. Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan;9. Research Center for Circular Economy, National Cheng Kung University, Tainan 701, Taiwan;10. College of Engineering, Tunghai University, Taichung 407, Taiwan |
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| Abstract: | Aqueous two-phase systems (ATPS) are recognized as very suitable techniques for the recovery of target solutes in biological applications. Three new phase diagrams of (UCON 50-HB-5100 + potassium citrate + water), (UCON 50-HB-5100 + sodium formate + water), and (UCON 50-HB-5100 + potassium formate + water) systems were measured at 23 °C. The binodal curves were successfully described using the empirical equation suggested by Merchuk and co-workers. The reliability of the tie-line data experimentally determined was evaluated using the equations reported by Othmer–Tobias and Bancroft and satisfactory linearity was obtained for all ATPS. Among the salts studied, potassium citrate proved to be the most effective in ATPS formation, providing the largest heterogeneous region. Besides, the effect of both anions and cations in the size of the heterogeneous region and in the slope of the tie-lines has been compared. For the same salts and conditions, the heterogeneous region using UCON as the phase-forming polymer is larger than using polyethylene glycol. Furthermore, laccase partition in the UCON-salt ATPS was studied and it was found that in all cases enzyme partition occurred preferably to the bottom phase (salt-rich phase). Laccase concentration in the salt-rich phase was approximately 2-fold that in the top phase, thus UCON-salt ATPS can be a suitable biphasic system for laccase extraction. |
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