Determination of Nucleic Acids Based on their Resonance Light Scattering Enhancement Effect on Metalloporphyrin Derivatives |
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Authors: | Email author" target="_blank">Zhanguang?ChenEmail author Weifeng?Ding Yizeng?Liang Fenglian?Ren Yali?Han Jinbin?Liu |
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Institution: | (1) Department of Chemistry, Shantou University, 515063 Shantou, China;(2) College of Chemistry and Chemical Engineering, Centralsouth University, 410083 Changsha, China |
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Abstract: | A new method for the determination of nucleic acids at nanogram per mL level is proposed based on the enhanced resonance light scattering (RLS) signal resulting from the interaction of metalloporphyrins with nucleic acids. Under optimum conditions, the weak RLS signal of metalloporphyrin is enhanced by nucleic acids, and the enhanced RLS intensity is proportional to the concentration of nucleic acids. The detection limits of calf thymus DNA were 3.5ngmL–1, 2.9ngmL–1 and 1.0ngmL–1 for three metalloporphyrins, respectively. Synthetic samples were determined with satisfactory results. |
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Keywords: | : Resonance light scattering (RLS) metalloporphyrin derivatives nucleic acids |
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