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Direct electrochemistry of glucose oxidase and sensing glucose using a screen-printed carbon electrode modified with graphite nanosheets and zinc oxide nanoparticles |
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| Authors: | Chelladurai Karuppiah Selvakumar Palanisamy Shen-Ming Chen Vediyappan Veeramani Prakash Periakaruppan |
| Institution: | 1. Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei, 106, Taiwan, Republic of China 2. Post Graduate and Research Department of Chemistry, Thiagarajar College, Madurai, 625009, Tamil Nadu, India |
| Abstract: | We have studied the direct electrochemistry of glucose oxidase (GOx) immobilized on electrochemically fabricated graphite nanosheets (GNs) and zinc oxide nanoparticles (ZnO) that were deposited on a screen printed carbon electrode (SPCE). The GNs/ZnO composite was characterized by using scanning electron microscopy and elemental analysis. The GOx immobilized on the modified electrode shows a well-defined redox couple at a formal potential of ?0.4 V. The enhanced direct electrochemistry of GOx (compared to electrodes without ZnO or without GNs) indicates a fast electron transfer at this kind of electrode, with a heterogeneous electron transfer rate constant (Ks) of 3.75 s?1. The fast electron transfer is attributed to the high conductivity and large edge plane defects of GNs and good conductivity of ZnO-NPs. The modified electrode displays a linear response to glucose in concentrations from 0.3 to 4.5 mM, and the sensitivity is 30.07 μA mM?1 cm?2. The sensor exhibits a high selectivity, good repeatability and reproducibility, and long term stability. Figure Graphical representation for the fabrication of GNs/ZnO composite modified SPCE and the immobilization of GOx |
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