Analytical performance of a multi-element ICP-AES method for Cd, Co, Cr, Cu, Mn, Ni and Pb determination in blood fraction samples |
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Authors: | Emmanouil J Daftsis George A Zachariadis |
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Institution: | (1) Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University, Thessaloniki, Greece |
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Abstract: | An analytical method using inductively coupled plasma atomic emission spectrometry (ICP-AES) for rapid simultaneous determination
of seven heavy metals (Cd, Co, Cr, Cu, Mn, Ni and Pb) in human blood fractions, like plasma, cells fraction and whole blood,
is performed. The optimum wavelength was selected using as criterions the sensitivity, the linearity and recovery of aqueous
standard solutions. The pretreatment of the sample, the centrifugal conditions, the necessity of digestion and the dilution
of the digests were also studied. For plasma it was possible to avoid digestion of the sample, but for cells fractions and
whole blood the digestion is necessary. The samples were acid-digested by HNO3 in closed Teflon tubes under high temperature and pressure conditions and were diluted before injection into ICP-AES. Also,
optimization of the inductively coupled plasma conditions like nebulizer argon flow rate, sample flow rate and power of radio
frequency was performed for each analyte. Finally, the effect of the type of the employed calibration technique on the total
variation of the method was examined. Calibration using the standard addition technique was proved more accurate for the determination
in terms of analyte recovery. The sensitivity and recovery (Cd 99%, Co 101%, Cr 100%, Cu 99%, Mn 101%, Ni 100% and Pb 97%)
of the developed method are presented for all examined blood fractions.
Correspondence: George A. Zachariadis, Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University,
Thessaloniki 54124, Greece |
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Keywords: | : Inductively coupled plasma atomic emission spectrometry metals blood fractions whole blood centrifugation |
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