Spectrophotometric determination of drugs in pharmaceutical formulations withN-bromosuccinimide and celestine blue |
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| Authors: | Chilukuri S P Sastry Kommula R Srinivas Kommuri M M Krishna Prasad |
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| Institution: | (1) Foods and Drugs Laboratories, Department of Organic Chemistry and Foods, Drugs and Water, Andhra University, 530 003 Visakhapatnam, India;(2) Department of Physical Chemistry, Andhra University, 530 003 Visakhapatnam, India |
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| Abstract: | Simple, sensitive and selective methods are proposed for the spectrophotometric determination of drugs, viz., tetracycline HCl, nifurtimox, ethionamide, propranolol HCl and isonicotinic acid hydrazide, based on their reactivity withN-bromosuccinimide (NBS). The method involves the addition of excess NBS of known concentration in the presence of 0.25M HCl and the unreacted NBS is determined by the measurement of the decrease in the absorbance of the dye celestine blue ( max 540 nm), which was found to be the most suitable of several dyes tested. This method was applied for the determination of drug contents in pharamaceutical formulations and enabled the determination of the drugs in microgram quantities (0.2 to 5  g/ml). Standard deviations were typically  0.5mg per dose (RSD 0.1–1.0%). No interferences were observed from excipients and the validity of the method was tested against reference method. Recoveries were 99.8–101.1%. |
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| Keywords: | tetracycline HCl nifurtimox ethionamide propranolol HCl isonicotinic acid hydrazide N-bromosuccinimide celestine blue spectro-photometry |
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