Gold nanoparticle mediated method for spatially resolved deposition of DNA on nano-gapped interdigitated electrodes,and its application to the detection of the human <Emphasis Type="Italic">Papillomavirus</Emphasis>
1.Institute of Nano Electronic Engineering,Universiti Malaysia Perlis,Kangar,Malaysia;2.School of Microelectronic Engineering,Universiti Malaysia Perlis,Kangar,Malaysia;3.School of Bioprocess Engineering,Universiti Malaysia Perlis,Arau,Malaysia
Abstract:
The authors introduce a method for spatially arranged DNA immobilization on 10-nm gold nanoparticles (GNP) deposited on a silicon substrate carrying nanogapped interdigitated electrodes. The GNPs are covalently bound to the surface via silane chemistry, and the single steps of fabrication are monitored by FTIR spectroscopy and atomic force microscopy. This GNP deposition technique is shown to reduce the size of the nanogaps to 130 nm. FTIR also was used to monitor the immobilization of DNA on the surface of the interdigitated electrodes. This method allows DNA to be immobilized in a uniform and homogenous way. The utility of the method is demonstrated by immobilizing probe DNA on the surface and detecting target DNA specific for the human papilloma virus via fluorescence with a detection limit as low as 1 pM. In our perception, this method for GNP-mediated DNA immobilization enables high-performance sensing of a wide range of target (analyte) DNA.
Graphical abstract Schematic presentation of gold nanoparticle-mediated and spatially resolved deposition of DNA on nano-gapped interdigitated electrodes. The method was applied to the chemiluminescent determination of the human papillomavirus
