Simultaneous determination of yohimbine and boldine by first-derivative synchronous spectrofluorimetry |
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Authors: | José Luis Vilchez Gonzalo Sánchez-Palencia Ramiro Avidad Luis Fermín Capitán-Vallvey Alberto Navalón |
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Institution: | (1) Department of Analytical Chemistry, University of Granada, E-18071 Granada, Spain |
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Abstract: | A method for the simultaneous determination of yohimbine and boldine in mixtures by first-derivative synchronous spectrofluorimetry has been developed. The method is based on their native fluorescence in 0.1N sulphuric acid medium. The constant wavelength difference chosen to optimize the determination was =em -em=82 nm. Yohimbine was measured at ex//em= 285/367 nm, and boldine at ex/em=272/354 nm. The range of application is 10–500 g/l for yohimbine and 1–50 g/l for boldine. The method was applied to the determination of yohimbine and boldine in synthetic mixtures and pharmaceuticals, with errors generally 2%. Relative standard deviations were about 2%.Dedicated to Professor Fermin Capitán on his 72th birthday |
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Keywords: | yohimbine boldine derivative spectroscopy synchronous spectrofluorimetry |
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