| 摘 要： | A new amperometric biosensor for hydrogen peroxide (H2O2) has been developed that is based on direct electrochemistry and electrocatalysis of hemoglobin (Hb) in a multilayer inorganic–organic
hybrid film. o-Phenylenediamine (PDA) was electropolymerized onto a glassy carbon electrode (GCE), and then negatively charged nanogold
particles and positively charged poly(diallyldimethylammonium chloride) (PDDA) were alternately assembled on the PDA/GCE surface.
Finally, Hb was electrostatically adsorbed on the surface of gold nanoparticles. The electrochemical behavior of the resulting
biosensor (Hb/{nanogold/PDDA}n/PDA/GCE) was assessed and optimized. The performance and factors influencing the biosensor were studied in detail. Under
optimal conditions, the immobilized Hb displayed good electrocatalytic response to the H2O2 reduction ranging from 1.3 μM to 1.4 mM with a detection limit of 0.8 μM (at 3δ). In addition, the biosensor exhibited rapid
response, good reproducibility, and long-term stability.
Electronic supplementary material to this paper is available in electronic form at
Correspondence: Dianyong Tang, Department of Chemistry and Life Science, Leshan Teachers College, Sichuan (Leshan) 614000,
P.R. China
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