1.Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering,Hubei University,Wuhan,China;2.Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials,Hubei University,Wuhan,China
Abstract:
A fluorescence assay is described for the fluorometric determination of dopamine (DA). It based on the use of silica-coated CdTe quantum dots (QD@SiO2). These were fabricated through a hydrothermal process. When DA is added to a solution of the QD@SiO2 and then oxidized by oxygen under catalytic action of tyrosinase to form dopamine quinone, the fluorescence of QD@SiO2 (acquired at excitation/emission wavelengths of 310/525 nm) decreases due to an electron transfer quenching processes. The assay has a linear calibration plot in the 0.05 to 30 μM DA concentration range and a 12.5 nM detection limit (at an S/N ratio of 3). The method was applied to the determination of DA in spiked human serum samples.
Graphical abstract Schematic presentation of a fluorometric dopamine (DA) assay by using silica-coated CdTe QDs (QD@SiO2). DA is oxidized by oxygen under catalytic action of tyrosinase to form dopamine quinone, and this causes the quenching of fluorescence of QD@SiO2 at excitation/emission wavelengths of 310/525 nm.
