Probe electrospray ionization of mixture solutions using metal needles with different tip conditions |
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| Authors: | Satoshi Ninomiya Shunpei Iwamoto Lee Chuin Chen Kenzo Hiraoka |
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| Institution: | 1. Interdisciplinary Graduate School, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511 Japan;2. Clean Energy Research Center, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511 Japan |
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| Abstract: | Time-dependent mass spectra of mixture solutions of cytochrome c and cholesterol were measured by probe electrospray ionization (PESI) mass spectrometry and the PESI characteristics for several metal needles were investigated with different tip conditions. For a mixture solution of cytochrome c and cholesterol in 0.1% formic acid methanol/water (1/1) at a motion frequency of 1 Hz, an acupuncture needle with a sharp tip and 0.5-mm-diameter titanium and stainless-steel wire needles gave strong cytochrome c and extremely weak cholesterol ion signals. When the frequency was lowered, and the high-voltage duration increased, the titanium and stainless-steel needles provided both cytochrome c and cholesterol ion signals with high intensities well separated over time. |
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