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Mass Spectrometric Analysis of Microcystins from Cyanobacterial Biomass: Optimization of the Sample Preparation Procedure |
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| Authors: | Е N Chernova Ia V Russkikh E I Afonina О А Keltsieva V A Ostrovskii Е P Podolskaya Z А Zhakovskaya |
| Institution: | 1.St. Petersburg Scientific Research Center for Ecological Safety,Russian Academy of Sciences,St. Petersburg,Russia;2.Institute for Analytical Instrumentation,Russian Academy of Sciences,St. Petersburg,Russia;3.St. Petersburg State Institute of Technology (Technical University),St. Petersburg,Russia |
| Abstract: | The sample preparation procedure for determination of microcystins from biomass by the LC–MS method was optimized. Treatment of freeze-dried biomass samples with acetonitrile prior to the main extraction was effectively used for decreasing the amount of protein-like matrix compounds in extracts. Use of acetonitrile–water mixtures during the main ultrasound-assisted extraction allowed increasing the total microcystins amount in the resultant extracts compared to the traditional extraction using 75% methanol. |
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