Detection of zinc ions by differential circularly polarized fluorescence excitation |
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Authors: | Dai Zhaohua Proni Gloria Mancheno Danny Karimi Sasan Berova Nina Canary James W |
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Institution: | Department of Chemistry, New York University, New York, New York 10003, USA. |
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Abstract: | A new chiroptical spectroscopic approach, differential circularly polarized fluorescence excitation (CPE), can be used to provide a selective method for detecting the presence of zinc ions. The approach utilizes the same instrumentation as fluorescence-detected circular dichroism and provides strong contrast in metal detection due to response to both chelation-enhanced fluorescence and circular dichroism upon metal ion binding. The observed contrast is therefore better than either of the parent spectroscopic detection methods. CPE also provides a strategy to reduce interference from background such as protein-based tryptophan fluorescence. |
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