Biomolecular interaction monitoring of autoantibodies by scanning surface plasmon resonance microarray imaging |
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| Authors: | Lokate Angelique M C Beusink J Bianca Besselink Geert A J Pruijn Ger J M Schasfoort Richard B M |
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| Institution: | Department of Biomolecular Chemistry, Nijmegen Centre for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University Nijmegen, P. O. Box 9101, NL-6500 HB Nijmegen, The Netherlands. |
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| Abstract: | A new commercial surface plasmon resonance (SPR) imaging analysis system with a novel SPR dip angle scanning principle allows the measurement, without the need for labeling, of the exact SPR dip angle. With this system hundreds of biomolecular interactions can be monitored on microarrays simultaneously and with great precision. The potency of this system is demonstrated by automatically monitoring the interactions between citrullinated peptides and serum autoantibodies of 50 rheumatoid arthritis (RA) patients and 29 controls in a single step. The smallest antibody concentration that could be measured in this experimental setup was 0.5 pM. |
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