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Design of a new electrogenerated polyquinone film substituted with glutathione. Towards direct electrochemical biosensors |
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| Authors: | S Reisberg A Korovitch V Noel LD Tran MC Pham |
| Institution: | a Laboratoire Interfaces-Traitements-Organisation et Dynamique des Systèmes (ITODYS), Université Paris-Diderot, associé au CNRS, UMR 7086, 15 rue Jean-Antoine de Baïf, 75205 Paris, France b Departamento de Quimica, Universidad Nacional de Rio Cuarto-Ruta 8, km601, 5800 Rio Cuarto, Argentina c Faculté de Technologie Chimique, Institut Polytechnique de Hanoi, 1 rue Dai Co Viet, C1-408, DHBK, Vienam |
| Abstract: | We developed a method to graft a tripeptide (glutathione) onto 5-hydroxy-1,4-naphthoquinone, an electropolymerizable molecule. The resulting thin conducting polymer presents a well-defined and stable electroactivity in neutral buffered solution, due to the embedded quinone group, and is able to covalently graft amino-modified DNA probe strands. It is shown that the bioelectrode presents positive current change following DNA hybridization. This makes a “signal-on” direct electrochemical DNA sensor. The results were obtained with low target concentration (50 nM) and the selectivity is excellent as a single-mismatch sequence can be discriminated from the full-complementary target. |
| Keywords: | Glutathione Conducting polymer Electrochemical DNA sensor Direct detection Oligopeptide grafting |
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