Determination of aminosaccharides by high-performance anion-exchange chromatography with pulsed amperometric detection

High-performance anion-exchange chromatography (HPAC) was used for the determination of aminosaccharides in microbial polymers, chitin, animal waste, sewage sludge, plant residues and soil. The aminosaccharides, galactosamine, mannosamine and glucosamine were separated on a strong anion-exchange column with 1OmM sodium hydroxide as the eluent and determined by pulsed amperometric detection (PAD). The HPAC-PAD methodology was compared with high-performance liquid chromatography (HPLC) with refractive index detection (RI) in terms of selectivity and sensitivity for aminosaccharides. The results indicate that HPAC-PAD required less sample preparation, and was more precise and nearly two orders of magnitude more sensitive than HPLC-RI. HPAC-PAD was not subject to matrix interferences and was highly selective for aminosaccharides. More than 3% of the total nitrogen in alfalfa, and 20% of that in straw, was found to be present as aminosaccharides.