Voltammetric and amperometric determination of 2,4-dinitrophenol metabolites |
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Authors: | Dejmkova Hana Stoica Anca-Iulia Barek Jiri Zima Jiri |
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Institution: | a Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, CZ-12843 Prague 2, Czech Republic b Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd, 030018 Bucharest 3, Romania |
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Abstract: | Methods for determination of 2-amino-4-nitrophenol and 4-amino-2-nitrophenol, metabolites of 2,4-dinitrophenol, were developed using differential pulse (DP) voltammetry and HPLC with amperometric and spectrophotometric detection. The applicability of these methods was tested by the determination of the analytes in model samples of urine after preliminary separation by solid-phase extraction. Voltammetry enabled parallel determination of both analytes, but its application in real matrix was severely limited due to the interference of other compounds present in urine. HPLC allowed the determination in real urine matrix down to micromolar concentrations; amperometric detection proved to be more sensitive and selective than the spectrophotometric one. |
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Keywords: | Carbon paste electrode Differential pulse voltammetry HPLC with amperometric detection 2 4-Dinitrophenol metabolites Aminonitrophenols |
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