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Development of interdigitated arrays coated with functional polyaniline/MWCNT for electrochemical biodetection: application for human papilloma virus |
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| Authors: | Tran Lam Dai Nguyen Dzung Tuan Nguyen Binh Hai Do Quan Phuc Nguyen Huy Le |
| Institution: | a Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Ha Noi, Viet Nam b Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Ha Noi, Viet Nam c Research Center for Environmental Technology and Sustainable Development, Hanoi University of Science, 354 Nguyen Trai Road, Ha Noi, Viet Nam d School of Chemical Engineering, Hanoi University of Science and Technology, 1 Dai Co Viet Road, Ha Noi, Viet Nam |
| Abstract: | In this study, polyaniline-multiwalled carbon nanotube film (PANi-MWCNT) has been polymerized on interdigitated platinum electrode arrays (IDA), fabricated by MEMS technology for the detection of human papillomavirus (HPV) infection, using immobilized peptide aptamers as affinity capture reagent. Label-free, electrochemical detection of the specific immune reaction between antigen peptide aptamer HPV-16-L1 (with a molecular weight of 1825 Da), the most common genotype in cytological normal women worldwide, and its specific antibody of HPV-16 (which is much bigger with molecular weight of ca. 150 kDa) on multifunctional PANi-MWCNT based arrays was reported. The most significant advantage of this technique consists of reagentless and multiple detection of antigen-antibody complex formation on well conducting IDA interface of PANi-MWCNT, without intermediate steps or any labeling reagents, as normally required in the previous works. |
| Keywords: | Interdigitated arrays (IDA) Polyaniline-multiwalled carbon nanotube film (PANi-MWCNT) Peptide aptamer-antigen affinity Electrochemical detection Human papilloma virus (HPV) |
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