A two-color-change, nanoparticle-based method for DNA detection |
| |
Authors: | Cao Y Charles Jin Rongchao Thaxton C Shad Mirkin Chad A |
| |
Institution: | Department of Chemistry and Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA |
| |
Abstract: | Herein, we describe the detailed synthesis of Ag/Au core-shell nanoparticles, the surface-functionalization of these particles with thiolated oligonucleotides, and their subsequent use as probes for DNA detection. The Ag/Au core-shell nanoparticles retain the optical properties of the silver core and are easily functionalized with thiolated oligonucleotides due to the presence of the gold shell. As such, the Ag/Au core-shell nanoparticles have optical properties different from their pure gold counterparts and provide another “color” option for target DNA-directed colorimetric detection. Size-matched Ag/Au core-shell and pure gold nanoparticles perform nearly identically in DNA detection and melting experiments, but with distinct optical signatures. Based on this observation, we report the development of a two-color-change method for the detection and simultaneous validation of single-nucleotide polymorphisms in a DNA target using Ag/Au core-shell and pure gold nanoparticle probes. |
| |
Keywords: | Two-color-change Oligonucleotides Detection Core-shell Nanoparticles |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|