Immobilization strategies of Brucella particles on optical fibers for use in chemiluminescence immunosensors |
| |
Authors: | Yael Liebes Robert S Marks Menachem Banai |
| |
Institution: | a Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel b Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel c The National Institute of Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel d The Ilse Katz Center for Meso and Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel e Department of Bacteriology, Kimron Veterinary Institute, P.O. Box 12, Bet Dagan 50250, Israel |
| |
Abstract: | Immunosensors are powerful analytical tools in clinical and veterinary diagnostics. This has led us to design a chemiluminescent immunosensor aimed at identifying anti-Brucella antibodies using optical fibers as the transducer. In order to develop the optimal transducer, to achieve an optimal chemical modification thereby allowing an optimal covalent binding of the protein receptor, several cleaning strategies and silane coupling agents were investigated. Brucella killed organisms were used as a model receptor for quantifying anti-Brucella IgG antibodies in a suspension compared to conventional colorimetric and chemiluminescent ELISA. A silane-benzophenone derivative was selected as the best performing silane coupling agent: the optical fiber immunosensor (OFIS) has showed the lowest limit of detection at 0.207 μg/ml, compared to 0.828 μg/ml and 0.414 μg/ml achieved by colorimetric and chemiluminescent ELISAs, respectively. These results, together with the additional advantages of rapidity, lower reagent volumes and moderate operating conditions, have set the grounds for further study in order to adapt this platform for on-site diagnostics of brucellosis disease markers. |
| |
Keywords: | Brucellosis Chemiluminescence (CL) Enzyme-linked immuno-sorbent assay (ELISA) Immunosensor Optical fiber immunosensor (OFIS) Silane coupling agent (SCA) |
本文献已被 ScienceDirect 等数据库收录! |
|