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LC-MS在体内药物分析方法学研究中常见的问题及其对策
引用本文:杨靖,丁黎,刘文英.LC-MS在体内药物分析方法学研究中常见的问题及其对策[J].中国科学:化学,2010(6):611-620.
作者姓名:杨靖  丁黎  刘文英
作者单位:药品质量与安全预警教育部重点实验室;中国药科大学药物分析教研室,南京210009药品质量与安全预警教育部重点实验室 中国药科大学药物分析教研室,南京210009
摘    要:液相色谱-质谱联用技术(liquid chromatography-mass spectrometry,LC-MS)已成为体内药物分析及其相关研究领域中不可或缺的工具.虽然该技术具有高选择性、高灵敏度以及高通量等特点,但在体内药物分析方法学研究中,仍然会面临诸如待测物需衍生化、复方制剂体内多组分同时测定时高浓度组分的质谱响应饱和、方法专属性误判、基质效应、残留效应等一系列挑战和问题,本文综述了这些常见问题的相应对策.

关 键 词:液相色谱-质谱联用  体内药物分析  衍生化  专属性  基质效应  残留效应

LC-MS in biomedical analysis:Common issues and counterplan
YANG Jing,DING Li & LIU WenYing.LC-MS in biomedical analysis:Common issues and counterplan[J].Scientia Sinica Chimica,2010(6):611-620.
Authors:YANG Jing  DING Li & LIU WenYing
Institution:Key Laboratory of Drug Quality Control and Pharmacovigilance,Ministry of Education;Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing 210009,China
Abstract:Liquid chromatography-mass spectrometry(LC-MS) is currently the method of choice for the biomedical analysis.The advantages of this technique include high specificity,sensitivity and throughput.However,in practical terms,we still face many challenges and/or problems,such as derivatization,MS response saturation to high content components in simultaneous determination of the multi-component,false-positive results of specificity,matrix effect and carry-over effect.In this review,these issues and their counterplan in bioanalytical LC-MS method development and validation are discussed and illustrated with some examples.
Keywords:liquid chromatography-mass spectrometry  biomedical analysis  derivatization  specificity  matrix effect  carry-over effect
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