曙红Y-Triton X-100疏水性氢键纳米微粒的吸收、荧光及共振瑞利散射光谱及其分析应用

在pH2.4～2.8的酸性介质中,曙红Y分子(H2L)取代水分子而与Triton X-100形成氢键缔合物.该疏水性的氢键缔合物,在水相的"挤压"作用和范德华力的作用下,能进一步聚集形成纳米微粒.此时将引起吸收光谱的变化和荧光猝灭,并导致共振瑞利散射(RRS)显著增强,为建立褪色分光光度法、荧光猝灭法和共振瑞利散射法测定Triton X-100创造了条件.三种方法均有较高的灵敏度.其中以RRS法灵敏度最高,对于Triton X-100的检出限为20.6ng/mL.本文研究了曙红Y与Triton X-100相互作用的适宜条件和对吸收、荧光和RRS光谱的影响.考察了共存物质的影响,表明方法有良好的选择性.发展和建立了灵敏、简便、快速测定Triton X-100的分光光度、荧光猝灭法和RRS新方法.文中还结合红外光谱、透射电子显微镜技术和量子化学方法对曙红Y-Triton X-100氢键缔合物及纳米微粒的形成以及对相应的光谱特性的影响进行了讨论,并研究了方法在环境分析中的应用.

Absorption,fluorescence and resonance Rayleigh scattering spectra of hydrophobic hydrogen bonding of Eosin Y/Triton X-100 nanoparticles and their analytical applications

In a weak acidic medium（pH 2.4？2.8）,Eosin Y molecules（H2L） could replace water molecules to associate with Triton X-100 to form hydrophobic hydrogen bonding complexes.These complexes could further aggregate to form nanoparticles through the squeezing action of the water phase and Van Der Waals force,resulting in changes in the absorption spectrum and fluorescence quenching of EY as well as the significant enhancement of resonance Rayleigh scattering.This enables the sensitive determination of Triton X-100 using the fading spectrophotometry,fluorescence quenching method and RRS method.Among them,the RRS method shows the highest sensitivity with a detection limit of 20.6 ng mL-1 for Triton X-100.The optimum experimental conditions and factors that affect the absorption,fluorescence and RRS spectra were tested.The effects of coexisting substances were investigated and the results showed good selectivity.Based on these results,new spectrophotometric method,fluorescence quenching method and RRS method for the determination of Triton X-100 were established.The hydrogen bonding association of Eosin Y with Triton X-100 and the formation of nanoparticles as well as their effects on related spectral characteristics were discussed utilizing infrared,transmission electron microscope technique and quantum chemical method.