大单室CdTe量子点脂质体的制备及其体内代谢途径

在水相中用谷胱甘肽(Glutathione,GSH)为稳定剂合成了量子产率为61%、发射峰为601.2nm的CdTe-GSH量子点,然后用大豆卵磷脂为膜,通过减压蒸发法合成了粒径在1.5μm左右大小的大单室CdTe量子点脂质体(GUVs-CdTe).GUVs-CdTe对量子点的包封率比其他量子点脂质体显著提高,可达86.3%.由静脉注射到小白鼠体内后,各组织切片的荧光显微照片表明GUVs-CdTe不能通过血脑屏障和气血屏障,主要被网状内皮系统去除,在脾脏和肝脏呈弥漫状分布,这和大单室脂质体静脉注射体内后的代谢完全一致.

Preparation of giant unilamellar CdTe quantum dot vesicles and their metabolic pathway in vivo

Glutathione （GSH） capped CdTe quantum dots （QDs） with photoluminescence quantum yields of 61% and the maximum emitting at 601.2 nm were prepared in water phase. Giant unilamellar CdTe quantum dot vesicles （GUVs-CdTe）,with diameters larger than 1.5 μm,were obtained using lower-pressure evaporation techniques with soybean lecithin. Compared with other QD liposome,the entrapment efficiency of GUVs-CdTe for QDs has been significantly improved to 86.3%. After GUVs-CdTe were injected in mice through the tail vein,the fluorescence microscopy of tissue sections showed that GUVs-CdTe could not pass through the blood-brain barrier and air-blood barrier,which were removed mostly by the reticuloendothelial system and were widely distributed in the spleen and the liver. The behavior is the same as the character of metabolic pathway of giant unilamellar vesicles by intravenous injections in mice.