| 人血清Alpha-1-酸性糖蛋白的糖基化分析 |
| |
| 引用本文： | 于龙,李秀玲,侯美倩,张秀莉,梁鑫淼.人血清Alpha-1-酸性糖蛋白的糖基化分析[J].中国科学:化学,2011(3):544-549. |
| |
| 作者姓名： | 于龙 李秀玲 侯美倩 张秀莉 梁鑫淼 |
| |
| 作者单位： | 中国科学院分离分析化学重点实验室中国科学院大连化学物理研究所; |
| |
| 基金项目： | 中国科学院知识创新工程重要方向项目(KSCX2-YW-R-170); 国家自然科学基金面上项目(20805046)资助 |
| |
| 摘 要： | 获取真实准确的蛋白质糖基化信息是全面了解糖基化修饰生物学功能的前提.针对简单蛋白质的糖基化分析通常采用反相高效液相色谱-串联质谱技术在肽的水平上对糖基化信息进行采集和解析.本文以人血清Alpha-1-酸性糖蛋白(AGP)酶解液为对象,发展了一套简单有效的蛋白质糖基化分析方法.本方法分为三个步骤,第一步是建立糖肽的理论m/z值表;第二步是获取糖蛋白酶解液的LC-MS谱图,并将每一个色谱峰中所包含糖肽的实际m/z值与理论m/z值进行人工匹配;第三步是对每个色谱峰的糖肽结构归属进行LC-MS/MS验证.采用本方法,我们从AGP酶解液中共鉴定出172条糖肽.与单独采用Survey模式的方法相比,本方法能够显著提高糖肽的覆盖率.
|
| 关 键 词： | 糖基化分析 Alpha-1-酸性糖蛋白 糖肽 质谱 |
Glycosylation analysis of human serum alpha-1-acid glycoprotein |
| |
| YU Long,LI XiuLing,HOU MeiQian,ZHANG XiuLi & LIANG XinMiao Key Lab of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian ,China.Glycosylation analysis of human serum alpha-1-acid glycoprotein[J].Scientia Sinica Chimica,2011(3):544-549. |
| |
| Authors: | YU Long LI XiuLing HOU MeiQian ZHANG XiuLi & LIANG XinMiao Key Lab of Separation Science for Analytical Chemistry Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian China |
| |
| Institution: | YU Long,LI XiuLing,HOU MeiQian,ZHANG XiuLi & LIANG XinMiao Key Lab of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China |
| |
| Abstract: | Protein glycosylation information is of great importance for elucidating the biofunctions of glycosylation modification. For the glycosylation analysis of simple glycoproteins, tandem mass spectrometry coupled with reversed-phase liquid chromatography (RPLC-MS and MS/MS) is a common technique for acquiring the glycosylation information in the glycoprotein digests. We have developed a simple and effective glycosylation analysis method by using human serum alpha-1-acid glycoprotein digest as a model glycoprot... |
| |
| Keywords: | glycosylation analysis AGP glycopeptides mass spectrometry |
| 本文献已被 CNKI 维普 等数据库收录！ |
