| 基质辅助激光解析电离-飞行时间质谱分析寡核苷酸G-四链体 |
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| 引用本文： | 宋玉玲,吕美玲,汪海林.基质辅助激光解析电离-飞行时间质谱分析寡核苷酸G-四链体[J].中国科学:化学,2011,41(1):74-81. |
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| 作者姓名： | 宋玉玲 吕美玲 汪海林 |
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| 作者单位： | 环境化学与生态毒理学国家重点实验室; 中国科学院生态环境研究中心, 北京100085 |
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| 摘 要： | 我们发展了一种利用基质辅助激光解析电离飞行时间质谱技术(MALDI-TOF MS)分析对金属离子具有较高亲和力的寡核苷酸G-四链体的方法.考察了不同基质:3-羟基吡啶甲酸(3-HPA)与柠檬酸氢二铵(DHC)混合基质、3,4-二胺基苯基苯甲酮(DABP)及DABP/DHC混合基质,应用于G-四链体分析的效果.实验结果表...
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| 关 键 词： | MALDI-TOF MS G-四链体 3-HPA DHC DABP 基质选择性 |
| 收稿时间： | 2010-01-04 |
| 修稿时间： | 2010-04-27 |
Analysis of G-quadruplexes by matrix assisted laser desorption/ionization time-of-flight mass spectrometry |
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| SONG YuLing,LU MeiLing & WANG HaiLin State Key Laboratory of Environmental Chemistry , Eco-toxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing ,China.Analysis of G-quadruplexes by matrix assisted laser desorption/ionization time-of-flight mass spectrometry[J].Scientia Sinica Chimica,2011,41(1):74-81. |
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| Authors: | SONG YuLing LU MeiLing & WANG HaiLin State Key Laboratory of Environmental Chemistry Eco-toxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China |
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| Institution: | SONG YuLing,LU MeiLing & WANG HaiLin State Key Laboratory of Environmental Chemistry and Eco-toxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China |
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| Abstract: | A matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method was developed for analysis of G-quadruplexes. Various matrixes and their combination including 3-hydroxypicolinic acid (3-HPA), 3,4-diaminobenzophenone (DABP), and diammnium hydrogen citrate (DHC) had been studied. The combination of DABP and DHC can improve the quality of MALDI-TOF MS analysis of PolyT, showing less fragmentation and alkali metal ion adduct. However, this combination cannot generate MS signal of G-quadruplexes. In comparison, the combination of 3-HPA and DHC can be applied for MALDI-TOF analysis of G-quadruplexes, with the low limit of detection of 10 fmol. |
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| Keywords: | MALDI-TOF/MSzz G-quadruplexeszz 3-hydroxypicolinic acid (3-HPA)zz 3zz 4-diaminobenzophenone (DABP)zz diammnium hydrogen citrate (DHC)zz selectivity of matrixeszz |
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