| 丁二酸酐修饰对漆酶稳定性和除酚效率的影响(英文) |
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| 引用本文： | 熊亚红,高敬忠,郑坚鹏,邓乃康.丁二酸酐修饰对漆酶稳定性和除酚效率的影响(英文)[J].催化学报,2011(10). |
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| 作者姓名： | 熊亚红 高敬忠 郑坚鹏 邓乃康 |
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| 作者单位： | 华南农业大学理学院生物材料研究所; |
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| 基金项目： | supported by the key Academic Program of the 3rd Phase211 Projectof South China Agricultura University(2009B010100001); the National Natural Science Foundation of China(20906034B0604); the State Bureau of Forestry Project(2011-4-75)~~ |
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| 摘 要： | 采用(NH4)2SO4分步沉淀法对诺维信中国公司生产的漆酶制剂DeniLiteIIS进行了纯化,并用丁二酸酐(SA)对纯化酶进行了化学修饰,运用三硝基苯磺酸法、紫外光谱法及荧光光谱法对修饰效果进行了初步表征,比较了天然酶和修饰酶的pH稳定性、热稳定性及除酚效率.结果表明,修饰酶的平均氨基修饰度为85%,其紫外吸收峰和荧光发射峰均出现蓝移,而且紫外吸收减小、荧光强度增加.尽管采用SA化学修饰未能改变漆酶的最适反应温度,但使其最适反应pH值由4.5提高到5.5,并且使酶活提高60%.与天然酶相比,修饰酶的pH稳定性和热稳定性更高,催化效率(kcat)和酶与底物的亲和力(kcat/Km)分别提高了53%和122%,对邻、间和对苯二酚的除酚效率分别提高了48%,57%和18%.这预示着这些修饰漆酶可望应用于工业生产和酚类污染废水的治理.
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| 关 键 词： | 丁二酸酐 漆酶 化学修饰 稳定性 动力学 除酚效率 |
Effects of Succinic Anhydride Modification on Laccase Stability and Phenolics Removal Efficiency |
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| XIONG Yahong,GAO Jingzhong,ZHENG Jianpeng,DENG Naikang Institute of Biomaterial,College of Science,South China Agricultural University,Guangzhou ,Guangdong,China.Effects of Succinic Anhydride Modification on Laccase Stability and Phenolics Removal Efficiency[J].Chinese Journal of Catalysis,2011(10). |
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| Authors: | XIONG Yahong GAO Jingzhong ZHENG Jianpeng DENG Naikang Institute of Biomaterial College of Science South China Agricultural University Guangzhou Guangdong China |
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| Institution: | XIONG Yahong*,GAO Jingzhong,ZHENG Jianpeng,DENG Naikang Institute of Biomaterial,College of Science,South China Agricultural University,Guangzhou 510642,Guangdong,China |
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| Abstract: | Chemical modification is a useful method to change the properties of enzymes.Laccase is a phenol oxidase belonging to a multicopper protein,which catalyzes the oxidation of many phenolics.DeniLite IIS,a commercial laccase preparation from the Novozymes China Company,was purified by ammonium sulfate fractional precipitation.Succinic anhydride(SA) was used as a modifier for the chemical modification of the purified laccase.The effects of modification were characterized using the 6-trinitrobenzene sulfonic aci... |
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| Keywords: | succinic anhydride laccase chemical modification stability kinetics phenolics removal efficiency |
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