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Fingerprints of Phalaenopsis Tissues in Growth and Spike Induction Periods—A Solid‐state 13C NMR Approach
Authors:Der‐Lii M Tzou  Lin‐Kai Ni  Mei‐Man Chen  Min‐Chuan Chiou  Li‐Chou Chen  Shan‐Te Hsu  Kuo‐Lung Ku  Chien‐Chung Cheng
Institution:1. Department Institute of Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC;2. Department of Applied Chemistry, National Chia‐Yi University, Chia‐Yi 60004, Taiwan, ROC;3. Department of Horticulture, National Chia‐Yi University, Chia‐Yi 60004, Taiwan, ROC
Abstract:Solid‐state Nuclear Magnetic Resonance (ss‐NMR) 13C single‐pulse excitation spectroscopy in combination with the magic‐angle spinning (MAS) technique was applied to a series of Phalaenopsis tissues, including the leaf, sheath, stem, and root, at different growth and spiking periods. Compared with{1H}/13C cross‐polarization MAS spectra, the 13C single‐pulse excitation MAS spectra displayed very distinct spectral patterns, recognizable as fingerprints of the tissues studied. 1Here, we demonstrate that solid‐state 13C single‐pulse excitation NMR spectroscopy provides a direct and robust analytical tool for studying the various tissues of Phalaenopsis in different growth and spiking induction periods.
Keywords:Solid‐state NMR  Phalaenopsis  Cross‐polarization  Spike  Magic‐angle spinning
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