Derivatization-free determination of aminoglycosides by CZE–UV in pharmaceutical formulations |
| |
Authors: | Tiago Augusto Donegatti Alnilan Lobato Eliana A R Duek Luís Moreira Gonçalves Elisabete Alves Pereira |
| |
Institution: | 1. Departamento de Física, Química e Matemática, Universidade Federal de São Carlos - UFSCar, Sorocaba, São Paulo, Brazil;2. Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo (USP), São Paulo, São Paulo, Brazil;3. Biomaterials Laboratory, Medical and Health Sciences Faculty, Pontifical University Catholic of São Paulo (PUC-SP), Sorocaba, São Paulo, Brazil |
| |
Abstract: | Aminoglycosides are a relevant class of antibiotics widely used by medics and veterinaries. There are a variety of reasons that make their determination relevant, such as quality control, environment and food contamination assessment, drug-release studies, among others. The lack of a chromophore makes aminoglycoside spectrophotometric detection particularly challenging, often requiring derivatization. In this work, an indirect detection method, making use of imidazole as a probe, applying CZE was successfully tested. It did not require derivatization, which simplified the sample preparation. Suitable figures of merit were obtained; recoveries between 95 and 105%, adequate repeatability and precision, correlation coefficients (r) above 0.998, and limits of detection (LODs) of 3.2 and 11 mg/L for gentamicin and paromomycin, respectively. As a proof-of-concept, it was also applied in a simple controlled release experiment that was well fitted using the Hill equation. |
| |
Keywords: | Aminoglycoside antibiotics Antibiotic determination Indirect detection Pharmaceutical sample Separation science |
|
|