Electrophoretic separation of multiple chiral center analyte with a three cyclodextrins mixture |
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Authors: | Zuo La Cécile Danel Gaëlle Grolaux Julie Charton Christophe Furman Emmanuelle Lipka |
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Institution: | 1. Univ. Lille, Inserm, Institut Pasteur de Lille, U1167 – Risk factors and molecular determinants of aging-related diseases, Lille, F-59000 France;2. Univ. Lille, CHU Lille, ULR-7365 – GRITA – Groupe de Recherche sur les formes Injectables et Technologies Associées, Lille, F-59000 France;3. Univ. Lille, Inserm, Institut Pasteur de Lille, U1177 – Drugs and Molecules for living Systems, Lille, F-59000 France |
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Abstract: | A capillary electrokinetic chromatography method (CEKC) was developed for complete stereoisomeric separation of a neutral, hydrophobic, multiple chiral center dihydropyridone analogue, a drug candidate proposed in type 2 diabetes treatment. A background electrolyte comprising three cyclodextrins was found to successfully separate the eight isomers. First an anionic cyclodextrin, the SBE-β-CD, was selected to allow the chiral separation of our neutral compound and partial resolutions of the eight isomers were obtained. Then, the effects of different parameters such as the nature and concentration of the other cyclodextrins added and pH of the buffer were examined. Finally, a triple CD-system consisted of 15 mM SBE-β-CD plus 15 mM γ-CD and 40 mM HP-γ-CD in a 50 mM borate background electrolyte at pH 10, was found to successfully separate the eight isomers. Last, the selectivity and limits of detection and quantification were evaluated for this optimized method. |
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Keywords: | Capillary electrophoresis Cyclodextrin Dihydropyridone Multiple chiral center |
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