In‐capillary detection of fast antibody‐peptide binding using fluorescence coupled capillary electrophoresis |
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| Authors: | Haifang Qin Shumin Ding Li Liu Yiwan Teng Yao Chen Cheli Wang Jinchen Li Jianhao Wang Pengju Jiang |
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| Institution: | 1. Institute of Pharmaceutical and Environmental Engineering, Changzhou Vocational Institute of Engineering, Changzhou, Jiangsu, P. R. China;2. School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China;3. School of Petrochemical Engineering, Changzhou University, Changzhou, Jiangsu, P. R. China |
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| Abstract: | Herein, we report a technique for detecting the fast binding of antibody‐peptide inside a capillary. Anti‐HA was mixed and interacted with FAM‐labeled HA tag (FAM‐E4) inside the capillary. Fluorescence coupled capillary electrophoresis (CE‐FL) was employed to measure and record the binding process. The efficiency of the antibody‐peptide binding on in‐capillary assays was found to be affected by the molar ratio. Furthermore, the stability of anti‐HA‐FAM‐E4 complex was investigated as well. The results indicated that E4YPYDVPDYA (E4) or TAMRA‐E4YPYDVPDYA (TAMRA‐E4) had the same binding priorities with anti‐HA. The addition of excess E4 or TAMRA‐E4 could lead to partial dissociation of the complex and take a two‐step mechanism including dissociation and association. This method can be applied to detect a wide range of biomolecular interactions. |
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| Keywords: | Anti‐HA Antibody‐Peptide Interaction CE‐FL YPYDVPDYA |
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