A two-dimensional electrophoresis map of Chinese hamster ovary cell proteins based on fluorescence staining |
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Authors: | Hayduk Eric J Choe Leila H Lee Kelvin H |
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Institution: | School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14850, USA. KHL9@cornell.edu |
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Abstract: | We report on the development of a detailed two-dimensional electrophoresis map of Chinese hamster ovary (CHO) cell proteins based on fluorescence staining and tandem time-of-flight (TOF/TOF)-mass spectrometry. We observed a 71% success rate in the identification of proteins even though the CHO genome is not sequenced. The map consists of 224 protein identifications present in 274 two-dimensional gel electrophoresis (2-DE) gel spots. We have also initiated a study of the phosphoproteome using a commercially available phosphoprotein-specific fluorescent stain. Using this stain, we observe 672 phosphorylated proteins, including many proteins known to be phosphorylated, which is 36% of the proteins we visualized with a total protein stain and consistent with expectations. |
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